Phenotypic diagnosis of esbl (extended-spectrum β-lactamas) enzymes and investigation of resistance gene existence (per) in the isolated klebsiella pneumonia bacteria of pulmonary patients ad mitted i
1. Islamic Azad University of Ayatollah Amoli 2. Islamic Azad University of Ayatollah Amoli 3. Islamic Azad University of Ayatollah Amoli
Abstract
Introduction
Producing β-lactamase enzymes by klebsiella pneumoniae, especially of esbl type, (extended-spectrum β-lactamas) is one of the health-care problems across the world. the prevalence of this enzymes varies in different geographic regions over time. this research aims to determine the prevalence of bacteria producing esbl. among klebsiella pneumoniae strains and trace the blaper gene in the shomale specialized hospital of amol.
Methods
The present descriptive-cross sectional study have done on 20 strains of isolated klebsiella pneumonia of experimental samples of patients reffred to shomal specialized hospital of amol in 2018, and identified by biochemical-differential tests. disc diffusion method was used to avaluate organism producing esbl identified by doing verified screeing and phenotypic tests. to identify per gene produced in resistant samples of klebsiella pneumoniae. pcr method was used.
Results
According to results of this study, 14 strains of positive esbl klebsiella pneumoniae identified pcr results for identifying strains containing 3 per genes showed that.
Conclusion
: results show that production of β-lactamase in the isolated bacteria at studied community accompany with high prevalence. the level of blaper gene was low. thus the production of β-lactamase among such isolates related to other kinds of β-lactamase.
