Synthesize nano systems containing curcumin as a chemotherapy drug with the aim of affecting ovarian cancer a2780s cell line

Najmeh alsadat Abtahi,1 Bibi fatemeh haghirosadat,2 Seyed mohammad moosavizadeh,3 Javad zavar reza,4,*

1. department of biochemistry, school of medicine, shahid sadoughi university of medical sciences(international campus)
2. department of advanced medical sciences and technologies, school of paramedicine, shahid sadoughi university of medical sciences
3. department of laboratory sciences, school of paramedicine, shahid sadoughi university of medical sciences
4. department of biochemistry, faculty of medicine, shahid sadoughi university of medical sciences

Abstract

Introduction

Cancer, also known as a malignant tumor or malignant neoplasm, is a group of 150 diseases involving abnormal cell growth with the potential to invade or spread to other parts of the body. ovarian cancer is the most lethal gynecological cancer and the fifth common cause of cancer mortality in women around the world. curcumin (c21h20o6) is a natural yellow compound which typically found in curcuma longa that is regarded as a natural polyphenolic antioxidant presented in many kinds of herbs. curcumin has been exhibited multiple therapeutic relevance including anti-cancer, anti-inflammatory, antioxidant, antimicrobial, anti-rheumatic, and hepatoprotective activities. in addition, poor absorption and rapid metabolism of curcumin severely limit its bioavailability. niosomes are the particles that can solve the problems like that. the purpose of this research is to synthesize nano system that containing curcumin with the aim of affecting ovarian cancer a2780s cell line.

Methods

Nanoniosomes containing tween 80, tween 60, cholesterol, dsp and curcumin synthesized by thin–film hydration method. entrapment efficiency (ee) is measured with spectrophotometer and also drug controlled-release from nanoparticles is evaluated by dialysis method. the average of size and zeta potential of nanoparticles are assessed with dls.

Results

Curcumin encapsulation in nanoniosomes was more than 90% and the drug release rate from nanocarriers showed an acceptable profile that was 29% in 48h in normal cell situation (37c and ph 7.4). the mean diameter of noisomes was smaller than 100nm and the zeta potential of them was positive.

Conclusion

In this study, a significant amount of curcumin encapsulated in niosomes. drug release rate from nanocarriers showed a controlled and time dependent profile. cationic potential shows that nanocarriers can entire cells easily.

Keywords

Ovarian cancer, curcumin, drug delivery, nanoniosome