Investigation the role of the tim3 and pdcd1 genes in the asthma treatment by the zataria multiflora boiss and carvacrol
Nasim Adabi,
1 Masoumeh heydari2,
2 Atiye eslahi2,
3 Mahsa mohajeri2,
4 Ali hatami bardar,
5 majid mojarad,
6,*
1. 1 Department of Biology, Faculty of Basic Sciences, Neyshabour University of Science and Research, Neyshabur, Iran
2. Department of medical Genetics, Faculty of medical science, University of Medical Sciences and Health Services, Mashhad,
3. Department of medical Genetics, Faculty of medical science, University of Medical Sciences and Health Services, Mashhad,
4. Department of medical Genetics, Faculty of medical science, University of Medical Sciences and Health Services, Mashhad,
5. 1 Department of Biology, Faculty of Basic Sciences, Neyshabour University of Science and Research, Neyshabur, Iran
Abstract
Introduction
Numerous medicinal and traditional uses for zataria multiflora and its constituent, carvacrol, have been described such as antispasmodic, anti-inflammatory, and antioxidant effects. in this study, effects of the hydroalcoholic extract of z. multiflora and carvacrol by the pdcd1 and tim3 pathways in moderate asthmatic disease were evaluated.
Methods
Forty eight asthmatic patients were selected according to gina guidelines and divided into four groups consisted of the placebo before the treatment (group 1), placebo after the treatment (group 2), the z. multiflora extract before treatment (5 and 10 mg / kg / day, z 5 and z 10 respectively) in the group 3, and the z. multiflora extract after treatment (5 and 10 mg / kg / day, z 5 and z 10 respectively) in the group 4, (n = 12 for each group). gene expression analysis was performed by the probe-based quantitative pcr technique.
Results
Using the carvacrol treatment without z. multiflora extract caused 29% increase in tim3 expression. in the other hand, this treatment reduces the expression of pdcd1 to 71% of the control level. according to these results, it seems the tim3 gene as an immune response inhibitor interfering with the asthma symptoms treatment by the carvacrol.
using the z. multiflora extract along with the carvacrol has no significant effect on the tim3 expression. on the contrary, the z. multiflora extract increases the expression of pdcd1 gene by 46%. this supports the immune response inhibitory role of the tim3 in the asthma symptoms treatment by the carvacrol.
the z. multiflora extract, alone, has no effect on the tim3 expression but it decreases the pdcd1 expression to the half.
Conclusion
The results of this study showed that the z. multiflora extract and carvacrol during two months treatment lead to tim3 and pdcd1 expression changes which effects on the immune regulatory pathways
Keywords
Asthma, pdcd1, tim3, respiratory inflammation, zatria multiflora, carvacro
